Agrodiagnostics.com / PCR test systems

PCR test systems

AgroDiagnostica, LLC has developed and implemented into practice a unique set of test systems based on polymerase chain reaction (PCR) for diagnosis and identification of a wide range of crop infectious diseases. Also developed were systems for nucleic acid isolation from plant material and preparation of samples for PCR analysis. Kits are manufactured in three different formats: analysis of PCR products by electrophoresis (E), analysis based on FLASH (FLuorescent Amplification-based Specific Hybridization) technology, and Real-Time PCR (Rt). With FLASH technology, fluorescence is registered with a PCR detector “Gene" or “Gene 4” ("DNA Technology" Research and Production Company, Russia), the results are displayed in 30-60 seconds. Sets based on FLASH technology do not require opening the tubes after amplification, which significantly reduces the risk of contamination. Reagent kits in the Rt format allow qualitative analysis and are adapted for such devices as DTprime, DTlite (DNA Technology, Russia), iQ5 iCycler (Bio-Rad Laboratories, USA). We also have kits adapted to Rotor-Gene 6000 (Qiagen Instruments, Germany).

The developed systems allow detecting and identifying pathogens very quickly, reliably and with high sensitivity.

Description Detection formats
Electro-phoresis FLASH Rt
Kits of reagents for detection of potatoes’ pathogens
Clavibacter michiganensis subsp. sepedonicus
Ralstonia solanacearum
Potato Leafroll Virus
Potato Virus M
Potato Virus S
Potato Virus X
Potato Spindle Tuber Viroid
Potato Virus Y
Potato Virus A
Potato Mop Top virus
Andean potato mottle virus
Andean potato latent virus
Globodera pallida
Globodera rostochiensis
Synchytrium endobioticum
Potato black ringspot virus
Potato actin mRNA
Kits of reagents for detection of sugar beets’ pathogens
Beet necrotic yellow vein virus
Pseudomonas syringae
Kits of reagents for detection of pathogens of cucumbers and tomatoes grown indoor
Xanthomonas euvesicatoria (earlier Xanthomonas campestris pv. vesicatoria type А)
Pseudomonas corrugata
Pseudomonas syringae pv. Lacrimans, (earlier Pseudomonas lacrimans)
Clavibacter michiganensis subsp. michiganensis
Pectobacterium carotovorum subsp. сarotovorum, Erwinia carotovora subsp. carotovora)
Ascochyta cucumis; Phoma cucurbitacearum; Didymella bryoniae

Ascochyta lycopersici, Didymella lycopersici

fungi of the genus Ascochyta (Didymella, Phoma)
Tomato ringspot virus
Tomato yellow leaf curl begomovirus new!
Tomato spotted wilt virus new!
Tomato brown rugose fruit virus soon!
Kits of reagents for detection of cereals’ pathogens
Septoria sp.
Septoria nodorum
Septoria tritici
Kits of reagents for detection of cereals’ pathogens
Fusarium sp.
Fusarium graminearum
Fusarium culmorum
Fusarium sporotrichioides
Fusarium langsethiae
Fusarium poae
Fusarium avenaceum
Fusarium tricinctum
Fusarium cerealis
Fusarium acuminatum
Fusarium torulosum
Fusarium solani
Kits of reagents for the detection of Fusarium fungi with similar profiles of the produced toxins
Toxins: DON, 3-ADON, 15-ADON, NIV, FUSX, ZEA
Primary producers: Fusarium graminearum, Fusarium culmorum, Fusarium cerealis
Toxins: T-2, HT-2, DAS, NEO, ZEA
Primary producers: Fusarium sporotrichiodes, Fusarium langsethiae
Toxins: NIV, FUSX, T-2, HT-2, DAS, ZEA
Primary producers: Fusarium poae
Toxins: MON, BEA, ENN A, B
Primary producers: Fusarium avenaceum, Fusarium tricinctum, Fusarium acuminatum, Fusarium torulosum
Kits of reagents for diagnosis of pathogens and pests of fruit crops
Plum Pox virus
Erwinia amilovora
Ceratitis capitata
Raspberry ringspot virus
Candidatus Phytoplasma Vitis new!
Candidatus Phytoplasma pyri new!
Candidatus Phytoplasma mali new!
Peach latent mosaic viroid new!
Peach rosette mosaic virus soon!
Kits of reagents for detection of quarantine’ pathogens
Clavibacter michiganensis subsp. sepedonicus
Erwinia amilovora
Ralstonia solanacearum
Plum Pox virus
Globodera pallida
Globodera rostochiensis
Bursaphelenchus xylophilus
Bursaphelenchus mucronatus
Pantoea stewartii subsp. stewartii
Ceratitis capitata
Beet necrotic yellow vein virus
Andean potato mottle virus
Andean potato latent virus
Diaporthe helianthi (Phomopsis helianthi)
Synchytrium endobioticum
Potato black ringspot virus
Raspberry ringspot virus
Tobacco ringspot virus
Tomato ringspot virus
Candidatus Phytoplasma Vitis new!
Candidatus Phytoplasma pyri new!
Candidatus Phytoplasma mali new!
Peach latent mosaic viroid new!
Tomato yellow leaf curl begomovirus new!
Tomato spotted wilt virus new!
Acidovorax citrulli new!
Impatiens necrotic spot tospovirus new!
Potato virus T
Potato yellowing virus
Chrysanthemum stem necrosis virus
Chrysanthemum stunt viroid
Potato yellow dwarf virus
Xanthomonas oryzae pv. Oryzae
Xanthomonas campestris pv. Campestris
Peach rosette mosaic virus soon!
Tomato brown rugose fruit virus soon!
Barley stripe mosaic virus, BSMV soon!
Wheat streak mosaic virus, WSMV soon!
Brome mosaic virus, BMV soon!
Maize dwarf mosaic virus, MDMV soon!
DNA/RNA extraction Kits
PREP-NA (DNA/RNA extraction)
PREP-GS
Reverse transcriptase