Agrodiagnostics.com / PCR test systems

PCR test systems

AgroDiagnostica, LLC has developed and implemented into practice a unique set of test systems based on polymerase chain reaction (PCR) for diagnosis and identification of a wide range of crop infectious diseases. Also developed were systems for nucleic acid isolation from plant material and preparation of samples for PCR analysis. Kits are manufactured in three different formats: analysis of PCR products by electrophoresis (E), analysis based on FLASH (FLuorescent Amplification-based Specific Hybridization) technology, and Real-Time PCR (Rt). With FLASH technology, fluorescence is registered with a PCR detector “Gene" or “Gene 4” ("DNA Technology" Research and Production Company, Russia), the results are displayed in 30-60 seconds. Sets based on FLASH technology do not require opening the tubes after amplification, which significantly reduces the risk of contamination. Reagent kits in the Rt format allow qualitative analysis and are adapted for such devices as DTprime, DTlite (DNA Technology, Russia), iQ5 iCycler (Bio-Rad Laboratories, USA). We also have kits adapted to Rotor-Gene 6000 (Qiagen Instruments, Germany).

The developed systems allow detecting and identifying pathogens very quickly, reliably and with high sensitivity.

Description Detection formats
Electro-phoresis FLASH Rt
Kits of reagents for detection of potatoes’ pathogens
Clavibacter michiganensis subsp. sepedonicus
Ralstonia solanacearum
Potato Leafroll Virus
Potato Virus M
Potato Virus S
Potato Virus X
Potato Spindle Tuber Viroid
Potato Virus Y
Potato Virus A
Potato Mop Top virus
Andean potato mottle virus  
Andean potato latent virus  
Globodera pallida
Globodera rostochiensis
Synchytrium endobioticum    
Potato black ringspot virus  
Potato actin mRNA
Kits of reagents for detection of sugar beets’ pathogens
Beet necrotic yellow vein virus  
Pseudomonas syringae  
Kits of reagents for detection of pea pathogens
Uromyces pisi  
Uromyces fabae  
Kits of reagents for detection of pathogens of cucumbers and tomatoes grown indoor
Xanthomonas euvesicatoria (earlier Xanthomonas campestris pv. vesicatoria type А)
Pseudomonas corrugata
Pseudomonas syringae pv. Lacrimans, (earlier Pseudomonas lacrimans)
Clavibacter michiganensis subsp. michiganensis  
Pectobacterium carotovorum subsp. сarotovorum, Erwinia carotovora subsp. carotovora)  
Ascochyta cucumis; Phoma cucurbitacearum; Didymella bryoniae

 

Ascochyta lycopersici, Didymella lycopersici

 

fungi of the genus Ascochyta (Didymella, Phoma)
 
Tomato ringspot virus  
Tomato yellow leaf curl begomovirus new!    
Tomato spotted wilt virus new!    
Kits of reagents for detection of cereals’ pathogens
Septoria sp.
Septoria nodorum  
Septoria tritici  
Kits of reagents for detection of cereals’ pathogens
Fusarium sp.
Fusarium graminearum  
Fusarium culmorum  
Fusarium sporotrichioides  
Fusarium langsethiae  
Fusarium poae  
Fusarium avenaceum  
Fusarium tricinctum  
Fusarium cerealis  
Fusarium acuminatum  
Fusarium torulosum  
Fusarium solani  
Kits of reagents for the detection of Fusarium fungi with similar profiles of the produced toxins
Toxins: DON, 3-ADON, 15-ADON, NIV, FUSX, ZEA
Primary producers: Fusarium graminearum, Fusarium culmorum, Fusarium cerealis
 
Toxins: T-2, HT-2, DAS, NEO, ZEA
Primary producers: Fusarium sporotrichiodes, Fusarium langsethiae
 
Toxins: NIV, FUSX, T-2, HT-2, DAS, ZEA
Primary producers: Fusarium poae
 
Toxins: MON, BEA, ENN A, B
Primary producers: Fusarium avenaceum, Fusarium tricinctum, Fusarium acuminatum, Fusarium torulosum
 
Kits of reagents for diagnosis of pathogens and pests of fruit crops
Plum Pox virus
Erwinia amilovora
Ceratitis capitata  
Raspberry ringspot virus  
Candidatus Phytoplasma Vitis new!    
Candidatus Phytoplasma pyri new!    
Candidatus Phytoplasma mali new!    
Peach latent mosaic viroid new!    
Kits of reagents for detection of quarantine’ pathogens
Clavibacter michiganensis subsp. sepedonicus
Erwinia amilovora
Ralstonia solanacearum
Plum Pox virus
Globodera pallida
Globodera rostochiensis
Bursaphelenchus xylophilus
Bursaphelenchus mucronatus
Pantoea stewartii subsp. stewartii
Ceratitis capitata  
Beet necrotic yellow vein virus
Andean potato mottle virus  
Andean potato latent virus  
Diaporthe helianthi (Phomopsis helianthi)
Synchytrium endobioticum    
Potato black ringspot virus  
Raspberry ringspot virus  
Tobacco ringspot virus  
Tomato ringspot virus  
Candidatus Phytoplasma Vitis new!    
Candidatus Phytoplasma pyri new!    
Candidatus Phytoplasma mali new!    
Peach latent mosaic viroid new!    
Tomato yellow leaf curl begomovirus new!    
Tomato spotted wilt virus new!    
Acidovorax citrulli new!    
Impatiens necrotic spot tospovirus new!    
DNA/RNA extraction Kits
PREP-NA (DNA/RNA extraction)
PREP-GS
Reverse transcriptase